1. A culture of cells infected with nasopharyngeal flushing from the patient was obtained. Under the microscope, irregularly shaped cells are seen, some of them are detached from the walls of the test tubes, intercellular ruptures are noted. Justify the data.

2. The nutrient medium before the culture of the cells and after incubation did not change the color of the indicator. Justify the color sample.

3. When viruses were cultured in a single-layer cell culture coated with a thin layer of agar, parts of virus-destroyed cells were found. Justify the changes.

4. A suspension of erythrocytes was added to the culture of cells infected with viruses, after contact and washing with a solution of sodium chloride, adherent erythrocytes remain on the surface of the virus-infected cells. Justify such changes.

Tests:

1. The interaction of the virus with the host cell, at which the viral NK is inserted into the cellular genome

A) integrative form

B) abortive form

C) phagocytosis

D) productive form

E) pinocytosis

2. Subordination of cellular, matrix-genetic mechanisms of one's own information is a unique form of expression of alien information

A) rickettsia and mycoplasma

B) chlamydia and viruses

C) only for viruses

D) mycoplasmas and viruses

E) only for rickettsia

3. The nonspecific stage of the adsorption of the virus on a sensitive cell occurs at the expense of

A) fusion of membranes

B) complementarity of receptors

C) lysogeny

D) proteins of the virus

E) intermolecular attraction

4. The path of penetration of the internal structure of the virion into the cytoplasm or nucleus of the host cell

A) phagocytosis

B) fusion of membranes

C) Invasion

D) Conversion

E) endocytosis

5. Replication of DNA-virus genomes is the synthesis of DNA molecules, takes place with the participation of cellular

A) endonuclease

B) DNA polymerase

C) transcriptases

D) revertase

E) RNA polymerase

6. The yield of complex virions from the cell occurs by

A) Adsorption

B) penetration

C) "explosion", destruction

D) budding

E) penetration

7. Contamination by extraneous microorganisms, the need for additional research to obtain a clean line of the virus being isolated - deficiencies in cultivation in

A) chicken embryo

B) transplanted culture

C) semi-transplanted culture

D) laboratory animals

E) non-transplantable culture

8. Method not applicable for virus identification

A) neutralization of cytopathic action

B) neutralization of the gemadsorption reaction

C) changing the appearance of the color sample

D) delayed hemagglutination reaction

E) Immobilization of viruses

9. The haemadsorption reaction is used in practice for

A) virus indication

B) differentiation of viruses

C) quantification

D) virus identification

E) qualitative definition

Lesson№ 7.

1. Topic: Microecology of the human body. Dysbacteriosis. Principles of restoration of the microflora of the human body. Gnotobiology.

2. Aim: To master the methods of sanitary-bacteriological evaluation of environmental objects of assessment, as well as qualitative methods for determining the composition of the microflora of the human body.

3. Objectives: To teach methods of studying the microflora of the environment, sanitary-bacteriological evaluation of water, soil, air, determining the composition of the microflora of the human body.

4. Main questions of the topic:

1. Normal human microflora, as a dynamic ecological system and the nature of the relationships in it.

2. Microflora of human skin, its qualitative and quantitative composition.

3. Microflora of the oral cavity, its main representatives.

4. Microflora of the esophagus, stomach and small intestine.

5. The microflora of the large intestine, its qualitative and quantitative composition. Value and influence on the macroorganism of the microflora of the large intestine.

6. Microflora of the urinary tract of a person.

7. Dysbacteriosis (dysbiosis) and the causes of its occurrence.

8. Laboratory diagnosis, correction and prevention of dysbiosis.

5. Methods of learning and teaching: Малые группы.

6. Literature:

1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.

2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.

3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.

4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.

5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.

6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.

7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.

8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.

9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.

In english language

1. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.

2. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4^th Edithion, 2008, UK, p.656.

3. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.

4. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.

Electronic resources:

1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).

2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).

3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).