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1. What should you do if you accidentally break a test tube with a microbial culture, and its contents will pour out onto the table, the floor, your clothes?

2. You have finished work in the bacteriological laboratory at the work table: a loop was placed in a tripod on a tripod, the spirit lamp was closed with a cap, the cap was removed from the head, a robe, work shoes were put on shoes, and they left the laboratory. What gross violations of personal hygiene and sanitary-epidemiological regime did you allow?

3. Students during the change in the training room took food. What gross violations of personal hygiene and sanitary-epidemiological regime did they allow?

4. To prepare the preparation for skimmed glass, a drop of isotonic sodium chloride solution is applied, into which the test material is applied and distributed evenly. The smear is dried in the air with a slide in the object. Then slowly spend 3 times through the flame of the burner. What stage is this process of smear preparation? Describe the correctness of the sequence of this process.

5. In a smear stained by Gram, red bands of medium size with rounded ends are found. Determine the morphology of microbes and the ratio of color to Gram.

6. In a smear stained by Gram, violet cocci in the form of grape clusters were found. Determine the morphology of microbes and the ratio of color to Gram.

Tests :

1). Biological microscopes with an immersion objective have a limiting resolution

A) 200 nm

B) 0.2 μm

C) 0.1 nm

D) 100 nm

E) 10 nm

2. Darkfield microscopy is based on the phenomenon of light diffraction, which can be achieved with the help of

A) electron microscope

B) of a conventional condenser

C) of a fluorescent microscope

D) the paraboloid or cardioid of the condenser

E) immersion microscope

3. In the immersion system of the microscope, the preparations

A) Hanging drop

B) the crushed drop

C) non-fixed smear

D) fixed stained smear

E) fixed smear

4. A scientist who proposed using aniline dyes in studying the morphology of bacteria

A) D. Ivanovsky

B) L. Pasteur

C) I. Mechnikov

D) R. Koch

E) A. Lewenhoek

5. Coloring method using ingredients: gentian violet, lugol solution, 96% ethanol and aqueous fuchsin

A) to Ozheshko

B) according to Zdrodovsky

C) according to Tsil-Nielsen

D) according to Romanovsky-Giemsa

E) by Gram

6. The organelle of bacteria that prevents phagocytosis

A) Capsule

B) dispute

C) cell wall

D) flagella

E) cytoplasm

7. The mesosome participates in

A) cell nutrition

B) cell division

C) capsule formation

D) cell movement

E) in cell transformation

8. The rigid structure of the microbial cell, which has a complex chemical composition, causes uniformity of form

A) Capsule

B) the membrane

C) cell wall

D) dispute

E) volutin

9. Microbial cells completely devoid of cell wall

A) spore-forming

B) Spheroplasts

C) protoplasts

D) capsule forming

E) stable

10. Cell having one flagella

A) monotrich

B) Peritrich

C) amphitrich

D) Lofotrich

E) L-form

11. Structure of the microbial cell that does not perceive the dyes

A) membrane

B) cell wall

C) dispute

D) Capsule

E) mitochondria

12. Substances that perform a reserve function (sulfur, oxalates, volutin granules, fat and glycogen)

A) disputes

B) inclusion

C) Capsules

D) cytoplasmic membrane

E) mitochondria

13. Structural component of a bacterial cell

A) lysosome

B) mitochondria

C) nucleus with nucleolus

D) nucleoid

E) endoplasmic reticulum

Lesson№3.

1. Topic: Morphological features of protozoa, microscopic fungi and viruses. Methods of microscopic study of protozoa, microscopic fungi and viruses.

 2. Aim: Learn the methods of studying the morphology and structure of viruses, fungi and protozoa.

3. Objectives: Teach how to prepare smears of "hanging" and "crushed" drops to study microorganisms in a living state.

1. Main questions of the topic:

1. Features of the level of organization of actinomycetes (streptomycetes), their similarity and difference from bacteria and fungi.

2. Morphology of mold fungi.

3. Principles of classification and morphology of protozoa.

4. The main properties of viruses.

5. Molecular-genetic organization of viruses, the basis of their taxonomy.

6. Types of the structure of viruses by the nature of packaging morphological subunits.

7. Infectious agents with unusual properties - viroids and prions.

 

5. Methods of learning and teaching: Small groups

6. Literature:

1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.

2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.

3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.

4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.

5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.

6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.

7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.

8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.

9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.

In english language

9. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.

10. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4th Edithion, 2008, UK, p.656.

11. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.

12. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.

Electronic resources:

1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).

2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).

3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).

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