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Нажимая кнопку "Продолжить", я принимаю политику конфиденциальности

1. To isolate the pure culture of anaerobes, the lab technician sowed the test material in a tube with Kitt-Tarozzi medium, taken directly from the refrigerator, followed by incubation in a thermostat. What mistakes in the technique of distinguishing pure culture are allowed?

2. During the outbreak of intestinal infections in Shymkent, the causative agents of dysentery, typhoid fever, cholera vibrio were isolated and identified from a group of patients. Justify, the selected pathogens with a specific microbiological term.

3. During the outbreak of cholera in the Keles region of the SKO in 1998, cholera vibrios were isolated at the same time from the reservoirs of patients and carriers. Justify these phenomena with a specific microbiological term.

4. To obtain a homogeneous bacterial population, a bacteriologist took part of an isolated colony on a dense nutrient medium in order to further study the properties of the grown microorganism. Justify this process with a specific microbiological term.

5. When sowing a bacterial culture on the Giessa medium, after a day of incubation, the color of the medium in the test tubes with glucose and in the float gas changed. What do these changes indicate?

6. When sowing the culture on the "motley" series 24 hours later in a test tube with peptone water, a clouding of the medium is observed - the indicator papers are one blistered and the other is blackened. Why?

7. Various carbohydrates (glucose, sucrose, rhamnose, etc.) are added to the nutrient agar to obtain the Gissa medium. The media were sterilized by a lab technician under pressure in one atmosphere. Justify the correctness or error in the preparation and sterilization of differential media with carbohydrates.

Tests:

11. The lag phase is

A) the phase of the onset of intensive growth

B) the phase of intensive division

C) the phase at which the number of bacterial cells does not increase

D) the phase at which the number of viable cells is constant and at the maximum level

E) the phase of bacteria death

2. Enzymes that catalyze the transfer of groups of atoms

A) hydrolases

B) transferases

C) lyase

D) isomerases

E) ligase

3. Enzymes that catalyze the cleavage reactions from the substratum of a chemical group not by hydrolytic routes

A) ligase

B) hydrolases

C) transferases

D) isomerases

E) lyase

4. Saccharolytic enzymes of a bacterial cell are detected by degradation

A) proteins

B) fats

C) carbohydrates

D) lipoproteins

E) gelatin

5. At the first stage of isolation of a pure bacterial culture, the following activities are carried out

A) the study of proteolytic properties

B) Smear microscopy

C) sowing on sloping nutritive agar

D) study of isolated colonies

E) seeding on the environment of Gissa

6. Apparatus used for growing microorganisms in the method of deep cultivation with aeration

A) microanoisate

B) thermostat

C) turbidostat

D) special reactor

E) chemostat

7. The metabolism of bacteria consists of

A) energy and transcription

B) constructive and broadcast

C) energy and constructive

D) transcription and translation

E) replication and transduction

8. The phase of logarithmic growth

A) the onset of intensive cell growth

B) the number of bacterial cells does not increase

C) the number of living cells at the maximum level

D) Intensive cell division

E) Bacteria death

9. For the cultivation of anaerobes in bacteriological laboratories,

A) distillers

B) anaerostats

C) Koch apparatus

D) Pasteur oven

E) autoclave

10. By source of carbon and type of food, bacteria are divided into

A) autotrophs and heterotrophs

B) metatrophs and aminoautotrophs

C) organotrophs and aminoheterotrophs

D) phototrophs and saprophytes

E) heterotrophs and parasites

11. Environments used to accelerate the detection and identification of bacteria belonging to a large number of species of enterobacteria and pseudomonas

A) differential-selective

B) special

C) synthetic

D) selective

E) semisynthetic

12. A method based on the joint cultivation of anaerobes with strict aerobes, in the cultivation of anaerobic bacteria

A) chemical

B) physical

C) combined

D) biological

E) mechanical

13. When protein is decomposed by some bacteria, hydrogen sulphide can be released, while the indicator paper is colored in

A) pink color

B) black color

C) blue color

D) yellow

E) the color does not change

14. In the second stage of the allocation of pure culture, the following activities

A) definition of sensitivity to phages

B) study of isolated colonies

C) study of antigenic properties

D) determination of sensitivity to antibiotics

E) infection of laboratory animals

15. Dissociation of aerobes by uniform distribution of bacterial loops on the surface of a dense nutrient medium

A) biological method

B) the physical method

C) chemical method

D) mechanical method

E) biochemical method

16. A selective medium

A) Saburo

B) Levina

C) alkaline agar

D) Endo

E) Kitta-Tarozzi

17. Environment for the cultivation of anaerobes

A) Ploskirev

B) Levina

C) Endo

D) Kitta-Tarozzi

E) Mueller

18. Nutrient media by composition of ingredients are divided into

A) Loose

B) liquid

C) dry

D) dense

E) natural

19. Nutrient media are liquid, semi-liquid, dense, loose and dry, they are divided into

A) complexity

B) designation

C) composition

D) consistency

E) the number

20. Nutrient media intended for selective isolation and accumulation of microorganisms of a certain type from materials

A) concentrating

B) the main

C) special

D) differential-diagnostic

E) elective

Lesson№ 5.

1. Topic: Isolation of a pure culture of aerobes and anaerobes (3.4 day of the study). Identification of bacteria. Biochemical properties of microorganisms. 

2. Aim: To master the methods of identification of pure cultures of bacteria used in the bacteriological diagnosis of infectious diseases.

3. Objectives: To teach on the basis of biochemical properties of identification of pure bacterial cultures used in the bacteriological diagnosis of infectious diseases.

4. Main questions of the topic:

1. The third stage of the allocation of pure culture.

2. Methods for the isolation of a pure culture of aerobic bacteria.

3. Methods for isolating the pure culture of anaerobic bacteria.

4. Determination of the cultural properties of bacteria in the laboratory for the purpose of identification or diagnosis.

5. Determination of the cultural properties of bacteria in the laboratory for the purpose of identifying or diagnosing them.

6. Determination of bacterial saccharolytic enzymes in the laboratory for the purpose of identification or diagnosis.

7. Determination of proteolytic enzymes of bacteria in the laboratory for the purpose of identification or diagnosis.

8. The fourth stage of the allocation of pure culture.

9. Effect of chemical factors on microorganisms.

10. Effects of physical factors on microorganisms.

11. The effect of biological factors on microorganisms.

5. Methods of learning and teaching: work in pairs

6. Literature:

1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.

2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.

3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.

4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.

5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.

6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.

7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.

8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.

9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.

In english language

17. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.

18. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4^th Edithion, 2008, UK, p.656.

19. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.

20. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.

Electronic resources:

1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).

2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).

3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).