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1. In a smear stained using the Romanovsky-Giemsa method, blue-violet crimped microorganisms with several large uneven curls are visible. What are these microorganisms?
2. In a smear stained by the method of Romanovsky-Giemsa microorganisms with numerous small curls in the form of the Latin letter "S" are seen, painted in a pink-lilac color. What are these microorganisms?
3. In the preparation of the "crushed" drop with the help of a darkfield method, microorganisms with uniform curls were found. What are these microorganisms?
4. In the preparation of the "crushed" drop, the filaments of the dissected septate mycelium are visible, which, interlacing, form a thick mycelium with one another, from which single-celled conidiophores exit, ending in a fan-shaped extension (conidia). What kind of marsupials are represented?
5. In the preparation of the "crushed" drop, the filaments of the septate mycelium are visible, from which the conidiophores leave in the form of a "brush". What kind of mushrooms are represented?
6. In the cytoplasm of the neurons of the deceased person, a round and oval form of formation (Babesh-Negri body) of red color was found on the ammon horn cut. For what disease are they characteristic?
7. In the study of sections from the cornea of the rabbit eye infected with vaccinia, microscopic examination in epithelial cells near the nuclei revealed inclusions of a rounded corpuscle. Which corpuscles found inclusions of a round-shaped corpuscle. What are the bodies found and what are they?
Tests:
1. To study what microorganisms are used drugs "crushed" and "hanging" a drop
A) colored
B) fixed
C) of the living
D) of live stained
E) of fixed stained
2. Acid-resistant microorganisms having the form of rods, branching cells
A) Spirochetes
B) actinomycetes
C) chlamydia
D) spirillae
E) Mycobacterium
3. Small intracellular microorganisms having different morphology depending on the stage of the intracellular development cycle
A) rickettsia
B) Mycoplasma
C) viruses
D) chlamydia
E) actinomycetes
4. Minor microorganisms of polymorphic form, not having a rigid cell wall
A) chlamydia
B) rickettsia
C) viruses
D) Mycoplasma
E) bacteria
5. Large Gram-negative bacteria with curls
A) rickettsia
B) Spirochetes
C) chlamydia
D) mushrooms
E) actinomycetes
6. Gram-positive bacteria having a mycelium of branching filaments
A) chlamydia
B) rickettsia
C) Spirochetes
D) mushrooms
E) actinomycetes
7. Unicellular microorganisms that have lost the ability to form a true mycelium
A) Yeast
B) viruses
C) Spirochetes
D) Rickettsia
E) chlamydia
8. Double-stranded RNA occurs only in
A) rickettsia
B) viruses
C) chlamydia
D) of mushrooms
E) bacteria
9. A slide with a well is used in the method
A) a thick drop
B) "crushed" drops
C) "hanging" drops
D) cultivation
E) coloring
10. Microorganisms in the host cell transforming into reticular bodies and only thus capable of division
A) rickettsia
B) viruses
C) chlamydia
D) Spirochetes
E) Mycoplasma
Lesson№ 4.
1. Topic: Nutrition, respiration, growth and reproduction of bacteria. Nutrient media and their classification. Scheme of the bacteriological method of investigation. The study of the growth pattern of various bacterial cultures on liquid and dense nutrient media. The release of a clean culture of aerobes and anaerobes. (1,2 day of the study).
2. Aim: To master the vital activity of microbial cells, the processes of their nutrition, respiration, growth, reproduction, patterns of interaction with the environment. To master the technique of preparation of nutrient media for the cultivation of microorganisms, methods for the isolation of pure cultures of aerobic and anaerobic bacteria, and methods for the identification of pure cultures of bacteria used in the bacteriological diagnosis of infectious diseases.
3. Objectives: To master the technique of seeding with a bacterial loop, a needle, a pipette, a spatula. Sow the test material on artificial nutrient media. To teach methods of preparing nutrient media, sowing isolated colonies of microorganisms on nutrient media in order to obtain a clean culture.
4. Main questions of the topic:
1. Metabolism - as a set of biochemical reactions,
flowing in the bacterial cell, the two sides of the metabolism.
2. Nutrition of bacteria.
3. Classification of bacteria by type of nutrition, energy sources.
4. Basic mechanisms of feeding bacteria.
5. Classification of bacteria by respiration type.
6. Growth and reproduction of bacteria.
7. Phases of reproduction of a population of bacteria in a liquid nutrient medium.
8. The main groups of enzymes of bacteria and their classification.
9. Growth factors.
10. The first stage of isolation of a pure bacterial culture.
11. Define specific microbiological terms: "species", "strain", "clone", "colony", "pure culture".
12. Strains of bacteria within one species, subdivided according to one characteristic (morphological, biological, pathogenic, antigenic, etc.).
13. Classification of nutrient media.
14. Requirements for nutrient media.
15. Universal nutrient media.
16. Elective (selective) nutrient media.
17. Differential-diagnostic environments.
18. Methods of sterilization of nutrient media.
19. Methods of cultivation of bacteria.
20. The second stage of the allocation of pure culture.
5. Methods of learning and teaching: Discussion and work in pairs
6. Literature:
1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.
2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.
3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.
4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.
5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.
6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.
7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.
8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.
9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.
In english language
13. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.
14. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4th Edithion, 2008, UK, p.656.
15. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.
16. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.
Electronic resources:
1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).
2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).
3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).
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