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1. In the training room, two Petri dishes with nutrient agar were left open for 60 minutes, followed by incubation in a thermostat at 37 ° C. the next day the number of colonies on both cups was 200 colonies. What is the name of this method of air research? What do the results show?

2. As a result of bacteriological examination of the vaginal secretion it was established: the reaction of the medium is acidic, a large number of Dodderlein sticks. What do the results show?

3. You are given the results of bacteriological analysis of drinking water, the total microbial number in 1 ml is 120, the coli-index is 4. Make a conclusion on these indicators, what measures need to be taken?

Tests:

1. Cohabitation of individuals of different species, in which one species benefits from symbiosis, without causing other harm

A) Commensalism

B) metabiosis

C) mutualism

D) satellism

E) parasitism

2. The intestinal microflora, participating in water-salt, protein, carbohydrate, cholesterol metabolism, performs the function

A) antagonistic

B) detoxification

C) antimutagenic

D) digestive

E) protective

3. Microorganisms constantly present in the macroorganism

A) non-permanent

B) optional

C) transient

D) optional

E) Resident

4. With a decrease in body resistance, representatives of normal microflora are able to cause purulent-inflammatory processes, called

A) reinfection

B) exogenous infection

C) autoinfection

D) superinfection

E) mixed infection

5. About stale fecal soil contamination is judged by the following microorganisms

A) Clostridium perfringence + enterobacteria

B) clostridia perfringence

C) E. coli + enterococcus

D) cytobacteria + enterobacteria

E) cytobacteria + colibacillus

6. Sanitary-microbiological state of water is estimated by

A) hemolytic streptococci + Staphylococcus aureus

B) microbial number + coli-index

C) cytobacteria + enterobacteria

D) perfringent titre

E) E. coli + enterococcus

7. The least amount of soil, expressed in grams, which contains one C. perfringens

A) the perfrigence index

B) the microbial number

C) colitis

D) coli-index

E) perfringence titer

8. Air research method based on filtration or suction through special filters, liquids

A) washings

B) sedimentary

C) filtration

D) aspirating

E) tampons

9. Sanitary-indicative microorganism of water

A) Staphylococcus aureus

B) hemolytic streptococcus

C) E. coli

D) Proteus

E) clostridium perfrigence

10. For the analysis of microbial contamination of tables, equipment, utensils, sanodezhdy, use the method

A) washings and tampons

B) sedimentary

C) aspirating

D) filtration

E) for Koch

Lesson№ 8.

1. Topic: Aseptic, antiseptic, disinfection and sterilization. Implementation and evaluation of effectiveness.

2. Aim: To master the methods of the effectiveness of disinfection and sterilization used in medicine.

3. Objectives: Teach methods of sterilization and disinfection.

4. Main questions of the topic:

1. Practical use of various temperature parameters in microbiological medicine.

2. Applied value in the microbiology of physical factors: the reaction of the medium (pH), humidity and drying, ionizing radiation, UV radiation, ultrasound and pressure.

3. The effect of chemicals on microorganisms, their use in the practice of a doctor.

4. Sterilization, its goals and methods.

5. Methods of disinfection.

6. Antiseptic.

7. Aseptic.

8. Methods for controlling the effectiveness of sterilization.

5. Methods of learning and teaching: дискуссия

6. Literature:

1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.

2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.

3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.

4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.

5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.

6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.

7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.

8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.

9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.

In english language

1. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.

2. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4th Edithion, 2008, UK, p.656.

3. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.

4. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.

Electronic resources:

1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).

2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).

3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).

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