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1. What should you do if you accidentally break a test tube with a microbial culture, and its contents will pour out onto the table, the floor, your clothes?
2. You have finished work in the bacteriological laboratory at the work table: a loop was placed in a tripod on a tripod, the spirit lamp was closed with a cap, the cap was removed from the head, a robe, work shoes were put on shoes, and they left the laboratory. What gross violations of personal hygiene and sanitary-epidemiological regime did you allow?
3. Students during the change in the training room took food. What gross violations of personal hygiene and sanitary-epidemiological regime did they allow?
4. To prepare the preparation for skimmed glass, a drop of isotonic sodium chloride solution is applied, into which the test material is applied and distributed evenly. The smear is dried in the air with a slide in the object. Then slowly spend 3 times through the flame of the burner. What stage is this process of smear preparation? Describe the correctness of the sequence of this process.
5. In a smear stained by Gram, red bands of medium size with rounded ends are found. Determine the morphology of microbes and the ratio of color to Gram.
6. In a smear stained by Gram, violet cocci in the form of grape clusters were found. Determine the morphology of microbes and the ratio of color to Gram.
Tests:
11. Prokaryotic cells
A) Bacteria
B) Protozoa
C) mushrooms
D) plant cells
E) animal cells
2. According to the new classification, gram-positive bacteria belong to the department of the kingdom of prokaryotes
A) Gracilikutes
B) Tenericutes
C) firmikutes
D) Mendozikutes
E) molikutes
3. Work with the cultures of the OOI in special laboratories is carried out with the permission of the Ministry of Health of the Republic of Kazakhstan
A) Groups I and II
B) Group III
C) IY groups
D) Groups II and II
E) I and IY groups
4. Discoverer of living microorganisms
A) D. Ivanovsky
B) L. Pasteur
C) I. Mechnikov
D) R. Koch
E) A. Lewenhoek
5. The device intended for steam sterilization under pressure
A) Pasteur oven
B) autoclave
C) Koch apparatus
D) water bath
E) centrifuge
6. Diplococci are located in the smear
A) singly
B) with the formation of bags, bales
C) as a chain
D) in the form of bunches of grapes
E) in pairs
7. In a Pasteur oven, sterilize
A) saline solution
B) rubber items
C) glassware
D) culture media
E) Synthetic materials
8. To date, microbiological laboratories are operating in accordance with the sanitary rules approved by the order of the Minister of National Economy of the Republic of Kazakhstan dated April 15, 2015 No. ....
A) 338
B) 15
C) 552
D) 10
E) 125
9. To microorganisms having a round, rod-shaped, convoluted and curved shape, ....
A) Bacteria
B) mushrooms
C) yeast
D) Protozoa
E) Viruses
1. To study the morphology of microorganisms, a method of investigation is used.
A) microscopic
B) microbiological
C) biological
D) Serological
E) genetic
2. The crimson form of bacteria is characteristic of ....
A) Spirochete
B) Staphylococci
C) Bacillus
D) Streptococcus
E) Sarcin
3. Microorganisms having different forms depending on the intracellular cycle of development include ....
A) chlamydia
B) Mycoplasma
C) viruses
D) mushrooms
E) Actinomycites
13. Minimal polimorfnye microorganisms that do not have a rigid cell wall:
A) Mycoplasma
B) rickettsia
C) viruses
D) chlamydia
E) bacteria
Lesson№ 2.
1. Topic: Ultrastructure of a bacterial cell. Methods of microscopic examination and coloration of microorganisms. Simple and complex methods of coloring.
2. Aim: To study the morphology and structure of bacteria, master the technique of preparation of smears and the technique of microscopy.
3. Objectives: To study the morphology and structure of bacteria, master the technique of preparation of smears and the technique of microscopy.
4. Main questions of the topic:
1. The structure of the bacterial cell.
2. Features of the cell wall (CW)of gram-positive bacteria.
3. Features of the cell wall (CW)of gram-negative bacteria.
4. L-transformation of bacteria - as the most important adaptive form of bacteria to unfavorable conditions.
5. Cytoplasm, the basic structural elements and macromolecules of the cytoplasm.
6. Periplasmic space - its role in the functioning of the cell.
7. Capsules of bacteria, their most important properties, methods of their detection during microscopy.
8. Flagellum, donor villi, pili, or cilia - their chemical structure, biological significance for a bacterial cell.
9. Endospores and spore formation - as a protective form of bacteria under adverse conditions of existence.
10. Noncultivated forms of bacteria (NFPs) in non-spore-forming microorganisms.
11. Preparation of drugs for studying microorganisms in their native form: methods of "hanging", "crushed" drops and vital (lifelong) color.
12. Order of Spirochaetalis, their morphological features. Intraspecific differentiation by morphological features.
13. Morphological features of rickettsia, their taxonomy, classification. Morphology of the vegetative and resting stages.
14. Morphological features of chlamydia their classification systematics. Morphology of elementary, reticular and intermediate chlamydia bodies.
15. Morphological features of mycoplasmas, their taxonomy and classification, the level of organization of mycoplasmas
16. The device of a light microscope.
17. Immersion microscopy.
18. Darkfield microscopy.
19. Phase contrast microscopy.
20. Fluorescent microscopy.
21. Electron microscopy.
22. Morphology of bacteria.
23. Preparation of fixed smear preparations.
24. Simple methods of coloring.
25. Stages and mechanisms of coloring on Gram.
26. Peculiarities of coloring by the methods of Ozheshka, Neisser, Burri-Hins, Tsil-Nielsen, Romanovsky-Giemsa.
6. Literature:
1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.
2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.
3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.
4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.
5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.
6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.
7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.
8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.
9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.
In english language
5. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.
6. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4th Edithion, 2008, UK, p.656.
7. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.
8. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.
Electronic resources:
1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).
2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).
3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).
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