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1. Six months after the mass death of cows, the remains of animal skins were taken for examination. The Ascoli thermocyclization reaction was used, with precipitating anthrax serum. When annulusrecipitation was taken into account, a white ring appeared at the boundary of the liquid after 5 minutes. What is this phenomenon? What is the use of ring precipitation for this purpose?

2. Rhesus-negative woman, who became pregnant for the first time from a Rh-positive husband, turned to a women's consultation. What kind of reaction should I put? What will be the results in this case?

3. In the women's consultation turned Rh-negative woman. In the past, there were only one birth, two miscarriages, one turn. In this case, the fifth pregnancy from a Rh positive parent. How can one detect the accumulation of incomplete antiresusive antibodies in the serum of a woman?

Tests:

1. In the Coombs reaction, antibodies are detected

A) monoclonal

B) polyclonal

C) normal

D) abzymes

E) incomplete

2. Serological reaction forming a turbidity, then a loose sediment when the antigen is mixed with an antibody

A) neutralization

B) flocculation

C) enzyme immunoassay

D) Immunofluorescence analysis

E) immunodiffusion

3. Lysis of cells, when immune complexes are formed, occurs in the reaction

A) flocculation

B) agglutination

C) precipitation

D) Immune Lysis

E) RSK

4. Binding of complement, with the formation of an antigen-antibody complex occurs in the reaction

A) agglutination

B) immobilization

C) neutralization

D) Precipitation

E) RSK

5. Detection of the antibody when the immune serum is mixed with the corresponding virus occurs in the reaction

A) complement fixation

B) precipitation

C) agglutination

D) neutralizing viruses

E) immobilization

6. Neutralization of antigens occurs in the reaction

A) RTGA

B) DGC

C) Koons

D) RIA

E) ELISA

7. Bonding of corpuscular antigens and their precipitation occurs in the reaction

A) Koons

B) neutralization

C) DGC

D) Precipitation

E) Immunofluorescence

8. Precipitation of the antigen present in the dispersed, colloidal state occurs in the reaction

A) Precipitation

B) agglutination

C) flocculation

D) Immune Lysis

E) complement fixation

9. The interaction of the antiserum with the antigen solution occurs in the reaction

A) Immunodiffusion

B) neutralization

C) immobilization

D) DGC

E) Immunofluorescence

10. Antitoxic immunity against diphtheria or scarlet fever is determined by the reactions of Schick or Dick, which are related to reactions

A) agglutination

B) precipitation

C) Immune Lysis

D) RNGA

E) neutralization

Lesson№ 17.

1. Topic: Immunoprophylaxis and immunotherapy of infectious diseases. Allergy. Hypersensitivity reactions.

2. Aim: To study IBMP, widely used for the prevention, treatment and diagnosis of infectious and non-infectious diseases.

3. Objectives: Form an idea of the receipt and use of vaccines, anatoxins, immune sera and immunoglobulins. To familiarize with the technique of setting skin-allergic tests for assessing cellular immunity.

4. Main questions of the topic:

1. Give the definition of the term "allergy". Types of SST and HZT allergies, their mechanism of action.

2. Sensitization and desensitization of the body. Give a characteristic of autoantigens, autoaggressive states.

3. Diseases related to congenital and acquired immunodeficiency states.

4. List the drugs used for immunoprophylaxis and immunotherapy of infectious diseases.

5. Application, classification of vaccines depending on the methods of preparation.

6. Properties of strains of microorganisms used for the preparation of live vaccines.

7. Infectious diseases, prevention of which is carried out with the help of killed corpuscular vaccines.

8. Obtaining and practical application of autovaccine.

9. Methods of producing anatoxins. Infections, prevention of which is carried out with the help of anatoxins.

10. Production and use of subunit vaccines. Adjuvants, their role in a complex of biologics.

11. Methods of application and production of therapeutic and prophylactic serums.

12. Methods of application and production of immunoglobulins.

13. Preparation and use of diagnostic sera.

14. Difference of adsorbed agglutinating sera from unadsorbed sera.

15. Biological preparations used for skin and allergic tests, their significance and diagnosis of infectious diseases.

Describe a positive reaction to the administration of an allergen.

5. Methods of learning and teaching: Situational challenges

6. Literature:

1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.

2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.

3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.

4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.

5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.

6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.

7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.

8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.

9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.

In english language

33. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.

34. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4^th Edithion, 2008, UK, p.656.

35. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.

36. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.

Electronic resources:

1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).

2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).

3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).