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1. In the training room, two Petri dishes with nutrient agar were left open for 60 minutes, followed by incubation in a thermostat at 37 ° C. the next day the number of colonies on both cups was 200 colonies. What is the name of this method of air research? What do the results show?

2. As a result of bacteriological examination of the vaginal secretion it was established: the reaction of the medium is acidic, a large number of Dodderlein sticks. What do the results show?

3. You are given the results of bacteriological analysis of drinking water, the total microbial number in 1 ml is 120, the coli-index is 4. Make a conclusion on these indicators, what measures need to be taken?

Tests:

1. 1. Cohabitation of individuals of different species, in which one species benefits from symbiosis, without causing other harm

A) Commensalism

B) metabiosis

C) mutualism

D) satellism

E) parasitism

2. The intestinal microflora, participating in water-salt, protein, carbohydrate, cholesterol metabolism, performs the function

A) antagonistic

B) detoxification

C) antimutagenic

D) digestive

E) protective

3. Microorganisms constantly present in the macroorganism

A) non-permanent

B) optional

C) transient

D) optional

E) Resident

4. With a decrease in body resistance, representatives of normal microflora are able to cause purulent-inflammatory processes, called

A) reinfection

B) exogenous infection

C) autoinfection

D) superinfection

E) mixed infection

5. About stale fecal soil contamination is judged by the following microorganisms

A) Clostridium perfringence + enterobacteria

B) clostridia perfringence

C) E. coli + enterococcus

D) cytobacteria + enterobacteria

E) cytobacteria + colibacillus

6. Sanitary-microbiological state of water is estimated by

A) hemolytic streptococci + Staphylococcus aureus

B) microbial number + coli-index

C) cytobacteria + enterobacteria

D) perfringent titre

E) E. coli + enterococcus

7. The least amount of soil, expressed in grams, which contains one C. perfringens

A) the perfrigence index

B) the microbial number

C) colitis

D) coli-index

E) perfringence titer

8. Air research method based on filtration or suction through special filters, liquids

A) washings

B) sedimentary

C) filtration

D) aspirating

E) tampons

9. Sanitary-indicative microorganism of water

A) Staphylococcus aureus

B) hemolytic streptococcus

C) E. coli

D) Proteus

E) clostridium perfrigence

10. For the analysis of microbial contamination of tables, equipment, utensils, sanodezhdy, use the method

A) washings and tampons

B) sedimentary

C) aspirating

D) filtration

E) for Koch

 

Lesson№ 13.

1. Topic: Infection, infectious process. Factors of pathogenicity of microorganisms: factors of adhesion and colonization, invasion, protection from phagocytosis, toxins. Virulence. Biological research method

2. Aim: To master the methods of biological research, widely used in the diagnosis of infectious diseases and scientific experiments..

3. Objectives: To teach methods of experimental infection and immunization of animals, bacteriological examination of corpses of dead animals. To give an idea of the biological method of studying the factors of pathogenicity and virulence, as well as methods for determining the virulence of bacteria and the activity of bacterial toxins.

4. Main questions of the topic:

1. Define the terms "infection", "infectious process", "infectious disease".

2. Forms of a manifest infection.

3. Forms of asymptomatic infection.

4. Pathogenicity of microorganisms, as a polydeterminant sign.

5. Virulence, units of its measurement.

6. Pathogenicity properties.

7. List the factors of pathogenicity of bacteria and give their characteristics.

8. Chemical nature, the main properties of exotoxins.

9. Methods for producing toxins, their practical importance and application.

10. The main properties and chemical nature of endotoxins.

11. Forms of infection and their characteristics.

12. Periods of infectious disease.

13. The main sources of infection.

14. Ways and methods of infection.

15. List and characterize the infectious properties of viruses, features of viral infections.

16. Methods, methods and objectives of experimental infection of laboratory animals.5. Methods of learning and teaching: дисскусия

6. Literature:

1. Medical microbiology, virologists and immunology. Ed. Academician of RAMS Zverev VV, Professor Boychenko MN - Moscow, GEOTAR-Media, 2010. - 1t. - 900 b.

2. Medical microbiology, virology and immunology. Ed. Vorobyeva A.A. - Moscow: MIA, 2008. - 690 p.

3. Medical microbiology, immunology and virology: a textbook for honey. universities. Ed. Korotyaeva AI, Babicheva SA - 5-еизд., Испр.и доп. - SPb .: SpecLit, 2012. - 760 pp. : ill.

4. Medical microbiology, virology, immunology. Ed. Borisova LB ..- M .: MIA, 2006.-734 p.

5. Medical Microbiology / Ch. Ed. Pokrovsky VI, Pozdeev O.K. - Moscow: GEOTAR MEDICINE, 2007.

6. Atlas on medical microbiology, virology and immunology: atlas / ed .: AS Bykov, AA Vorob'ev, VV Zverev. - 2 nd ed., Ext. - M .: Honey. inform. agency, 2008. - 272 p .: ill.

7. A guide to practical exercises on medical microbiology, virology and immunology. Ed. Tetz V.V. -М.: Медицина, 2002.-352 с.

8. Computer program "Diamorph" - "Medical microbiology" - atlas-guidance on bacteriology of mycology, protozoology and virology, edited by acad. prof. Vorobyeva A.A.

9. The determinant of bacteria Berdzhi / Ed. D. Houlta, N. Krig, P. Snitai et al., Moscow: Mir, 1997. - In 2 volumes.

In english language

21. Microbiology Fundamentals: A Clinical Approach - Standalone book 2nd Edition.

22. Richard V Georing, Hazel M Docrell, Mark Zukerman, Derek Wakelin, Ivan M Roit, Cedric Mims, Peter L Chiodini «Medical Microbiology», 4th Edithion, 2008, UK, p.656.

23. Jacquelyn G Black «Microbiology», 7 th ,WILEY, 2010, p. 846.

24. Patric R Muray, Ken S Rosenthal, Michael F Pfaller «Medical Microbiology», 5th Edithion, 2008, p. 962.

Electronic resources:

1. Medicine 3 year [Electrones resource]: a course of lectures. - Electron. text dan. (24.0 MB). - M: Publishing house "Balance", 2005. -1 эл. opt. disk (CD-ROM).

2. Medical microbiology ATLAS - a guide to bacteriology, mycology, protozoology and virology [Electron resource]: for stud. and doctors / ed. A.S. Bykov. -M., 2001. -1 el. opt. disk (CD-ROM).

3. Workshop for laboratory studies on general microbiology [Electron resource]: teaching method. Manual / R.T. Dzhanabaev [et al.] -Sh., 2004. -1 e. opt. disk (CD-ROM).

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